Prestained protein ladder western blot (2024)

Kaleidoscope Standards are a mixture of ten recombinant proteins in four colors (blue, pink, yellow, and green 10–250 kD), enabling easy gel and blot orientation via the multicolor pattern. Dual Xtra Standardsĭual Xtra Standards consist of 12 recombinant proteins between 2 and 250 kD all others consist of 10 recombinant proteins between 10 and 250 kD (note that the 2 kD band is not visible on fluorescent blots).

Improved Dual Color Standards are now brighter than the competition on both gels and blots. The overall brightness of Precision Plus Dual Color Standards has been increased for visual robustness throughout the entire protein electrophoresis and western blotting workflow.

• Stronger band intensity throughout blot development.
• Sharper for more accurate molecular weight estimation.
• Two orange reference bands at 70 kDa and 25 kDa and one green reference band at 10 kDa highlight the blue-stained protein ladder. All markers are bound by the secondary antibody and the bands. The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed.
• Brighter for easier identification of target protein The PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250 kDa) for use in protein electrophoresis (SDS-PAGE) and western blotting. The AcuteBand Pre-stained Protein Ladder is also ideal for Western Blotting by the LICOR System.
• All four standards have the same gel migration patterns, with three high-intensity reference bands (25, 50, and 75 kD). Precision Plus Protein™ Prestained Standards are available in Dual Color, All Blue, Kaleidoscope™, and Dual Xtra options. Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. Read their story and request a free set of matching (temporary) tattoos here. BOX 5199, Limerick, Pennsylvania, USA.Two students celebrated their love of science with Precision Plus Protein Kaleidoscope Ladder tattoos. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. For detection of any western blot, it is desirable to use prestained molecular weight markers (also called protein ladders) that are transferred to the membrane along with the protein sample. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. All claims must be made within 30 days following the date of delivery. Each lot of prestained protein standards is individually calibrated for estimating the MW of sample proteins. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. Prestained natural protein standards for SDS-PAGE and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to assess transfer efficiency on blots. All properties listed are typical characteristics and are not specifications. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. Im looking for a prestained protein marker to run alongside my samples on SDS-PAGE. Covalent attachment of a hydrophobic dye to the. protein I am trying to identify is quite large-about 300kda. Prestained ladders contain chemically modified proteins. During electrophoresis, the polypeptides of the ladder show up in the gel as distinctly colored bands.

  

  What this means is that before even loading the ladders into the gel for electrophoresis, these ladders are previously stained and ready to go. This does not indicate that the protein(s) of interest did not transfer fully. GangNam-Stain Prestained Protein Ladder is very suitable for protein separation from SDS-PAGE, confirmation of Western blot transfer efficiency, CBB staining. In a prestained protein ladder, each of these bands, or polypeptides, are already stained with a dye. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Prestained molecular weight protein ladders can often remain in the gel after transfer or can even migrate better than some samples proteins due to the dyes used. Please contact a technical service representative for more information. This product is for research use only and is not intended for therapeutic or diagnostic applications.