Azura Genomics on LinkedIn: #electrophoresis #westernblotting #sdspage #dna #poweringbetterpcr (2024)

Happy Juneteenth!Today we celebrate Freedom Day, a momentous event in our history marking the emancipation of enslaved African Americans. As we honor this day, let us reaffirm our commitment to fostering an inclusive society where everyone has the opportunity to thrive.#Juneteenth #FreedomDay #EmancipationDay #Equality

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Introducing AzuraView™ Probe Fast qPCR Mix Blue, an inhibitor-tolerant 2x master mix for use in real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan® and molecular beacon probes. The 2x master mix includes a blue tracer dye providing an easy-to-see, easy-to-pipette format. AzuraView™ Probe Fast qPCR Mix Blue reliably quantifies any DNA template including cDNA, genomic DNA, and low copy number viral DNA sequences. The ready-to-use 2x master mix delivers an increased limit of detection with best-in-class sensitivity, and the inert blue dye simplifies reaction setup and reduces pipetting error. The advanced 2x formulation allows efficient amplification from GC-rich templates and AT-rich templates, as well as resistance to many common PCR inhibitors.#TaqManProbe #MDx #GeneExpression #qPCR #PoweringBetterPCR

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Overcoming challenges and obstacles in RT-qPCR typically requires an optimization of the cDNA synthesis step to ensure sensitivity and good dynamic range. Here are four points to consider in order to reduce variation and low sensitivity of reverse transcription:1.Inhibitors of reverse transcription can lower cDNA yield – Optimize the RNA isolation and use a robust cDNA synthesis mix2.RNA degradation will compromise results – Avoid freeze/thaw of RNA samples and always include an RNase inhibitor3.Complex RNA secondary structure can lead to poor cDNA synthesis – Perform cDNA synthesis at 50°C to improve reverse transcription4.Variation can be introduced due to inconsistent pipetting and liquid handling – minimize pipetting steps by using a cDNA synthesis master mix and buffer conditions optimized for a wide range of input RNA#RTPCR #MolecularBiology #qPCR #DNA #PoweringBetterPCR

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Coming Soon - AzuraView™ Probe Fast qPCR Mix Blue, the Ultra-Sensitive Real-Time PCR Master Mix for TaqMan® Probe Assays.AzuraView™ Probe Fast qPCR Mix Blue is an inhibitor-tolerant 2x master mix for use in 2-step real-time quantitative PCR assays and has been formulated for probe-detection technology, including TaqMan® and molecular beacon probes. The 2x master mix includes a blue tracer dye providing an easy-to-see, easy-to-pipette format.For complex multiplex reactions that include Cy5 and Texas Red, AzuraView™ Probe Fast qPCR Mix is also available in a colorless version.#RTPCR #GeneExpression #PCR #TaqMan #PoweringBetterPCR

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Skip DNA Purification.Go direct from sample to PCR with our Flash-Extract™ PCR Kit. It provides a low-cost and highly efficient method of DNA extraction and amplification from a variety of solid tissues, including mouse tails, plant leaves, bacteria and saliva.Get PCR-Ready DNA from tissue samples in only 8 minutes. Extract and amplify DNA in less than 1 hour.#Genotyping #DNAExtraction #PCR #DNA #PoweringBetterPCR

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The AzuraQuant™ Probe Fast qPCR Mix is comprised of Azura HS Taq DNA Polymerase and an optimized buffer chemistry providing robust real-time PCR and broad range detection for increased sensitivity, speed, and reproducibility. In addition, the 2x master-mix exhibits excellent sensitivity in complex multiplex reactions, in which multiple amplicons can be detected without a loss in efficiency. •Probe-based detection of DNA and cDNA•TaqMan® gene expression analysis•Two-Step RT-qPCR•Multiplex qPCRAzuraQuant™ Probe Fast qPCR Mix is available in HiRox, LoRox, and NoRox versions for compatibility with all PCR devices. #qPCR #RTPCR #TaqManProbes #DNA #PoweringBetterPCR

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As we approach Memorial Day, our team at Azura Genomics thanks all veterans and their families for their service and sacrifice. We honor and celebrate all those who served and recognize the many lives that were lost in order to build a better tomorrow.#MemorialDay2024 #Veterans #RememberandHonor

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The MyGo Mini S and our AzuraView GreenFast qPCR Blue Mix is a powerful and cost-effective combination for ultra-fast and accurate gene expression analysis for 2-Step and 1-Step SYBR® Green qPCR detection workflows. Contact us to learn more or request a demo in your lab.#PCR #GeneExpression #qPCR #MDx #PoweringBetterPCR

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Our high-performance PCR reagents are now available in new product packaging designed to reduce waste and plastic, minimize cost during shipping and provide long term stability during cold storage. Our user-friendly box designs are available now.#PCR #qPCR #MolecularBiology #Reagents #PoweringBetterPCR

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